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CAS NO: 77-86-1
Molecular Formula: C4H11NO3
Molecular Weight: 121.14
EINECS NO: 201-064-4
MDL NO: MFCD00004679
Product Name: Tris(hydroxymethyl)aminomethane CAS 77-86-1
Assay : Greater than or equal to 99.0 percent
Density: 1.35g/cm3
Melting point:167-172 degree
Boiling Point:357 degree at 760 mmHg
Flash Point:219-220 degree
Tris is a weak base, with a pKa of 8.1 at 25 degree ; According to buffering theory, the effective buffering range of Tris buffer is between pH 7.0 and 9.2. The pH of Tris alkali aqueous solution is around 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value to obtain a buffer solution with that pH value. However, attention should also be paid to the effect of temperature on the pKa of Tris.
1. Tris is a weak base, and using this buffer system alone can prepare a buffer solution with a wide range of pH values from acidic to alkaline;
2. There is minimal interference with the experimental results and no precipitation occurs with calcium, magnesium ions, and heavy metal ions.
At room temperature (25 degree ), the effective buffering range of the buffer solution is between pH 7.0 and 9.2.
Tris buffer solution: pH{{0}}.5~9.0;
Tris HCl buffer solution: pH=7.5~8.5;
Tris phosphate buffer solution: pH{{0}}.0~9.0.
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